As the site of photosynthesis,
the chloroplast is the defining organelle of green plants and may be
thought of as the world’s
life-support system. We have chosen it for functional genomics studies
because it produces many molecules important to agriculture and human
health yet is similar in complexity to a bacterial cell.
Recent publications from the project:
Ajjawi, I., Y. Lu, L.J. Savage, S.M. Bell and R.L. Last. 2009. Large scale reverse genetics in Arabidopsis: Case studies from the Chloroplast 2010 Project Plant Physiol. Published online 11 Nov. PMID: 19906890.
To read the manuscript click here.
Gao* J., I. Ajjawi*, A. Manoli, A. Sawin, C. Xu, J. Froehlich, R. L. Last and C. Benning 2009. FATTY ACID DESATURASE 4 of Arabidopsis encodes a protein distinct from classic fatty acid desaturases. Plant J.
To read the paper click here.
(updated 12/09)
The pipeline continues to accept new homozygous lines as they become available and a new version of the data analysis tools database is being tested by project members prior to release.
To take advantage of updated in silico and experimental analysis we have expanded the universe of genes that are being included in our analysis. These genes are from stromal proteomics data from Klaas van Wijk at Cornell University and annotation made available from the SubCellular Proteomic Database (SUBA) at the Plant Energy Biology Centre of Excellence in Australia.
The gene list can be found elsewhere on this website.
Click to Learn More about the Project
Phenotypic data has been released
Phenotypic
data from the project for >3,500 mutants has been released. Please click here and establish a user account to access the data using our data analysis tools.
Protocols that we use for this project are accessible through this website. For more information click here.
Do you have homozygous mutant lines for known or putative plastid targeted proteins? We would like to include your mutants in our pipeline. Please contact Rob Last.
Summer Internships Available:
The fifth annual Plant Genomics @ Michigan State University Summer Undergraduate Research Program is accepting applications for summer of 2010 (until 12 March 2010). For information click here. To download the application click here.
Chloroplast 2010 is a collaborative Arabidopsis functional genomics project funded by a National Science Foundation grant to Michigan State University.
